Molecular Systems Core
We want to be part of your research project and we want to help you publish! We specialize in DNA detection methods, as well as a few others. Our prices are highly negotiable and can be as low as $5/sample. We are non-profit and we only seek moneys to cover cost. Highly trained and supervised graduate students gain valuable research experience while helping you complete your research. It is a "win-win" collaboration. Cost-sharing is often an option. Let us be your "lab-rats"!
Our molecular skills include:
- Quantitative PCR (i.e., serial dilution/end-point PCR)
- Quantitative Real-Time PCR
- Reverse-transcription PCR
- DNA cloning systems
- Protein production
- Antibody generation
- Immunofluorescence microscopy
- Western blotting
- DNA sequencing analysis
We have experience performing DNA detection on:
- Swabs in EtOH
- Water samples
- Tissue samples (e.g., biopsies, blood, toe-clips, tail-snips, rodents/birds/amphibians/reptiles)
- Scotch tape
- Whole insects (e.g., mosquitoes, parasitic worms, ticks)
More importantly, we know how to trouble shoot and have proper controls so you can feel confident in our results.
- spiked DNA positive-controls (reveals DNA inhibitors which lead to false-negative results)
- serial dilution controls (reveals minimum detection limits)
- typical negative controls (reveals the likelihood of false-positive results)
- always performed in triplicate (if not more)
- always visualize results on a gel (every wonder what that signal in qRT-PCR means?)
- always DNA sequenced for maximum reliability (just a few samples)
O’Bryan C.J.; Gray M.J.; Brooks C.S. 2012. Presence of Ranavirus Infection in Amphibian Populations of Tennessee, USA. Herpetological Review 43(2), 293–295.
- In this project, amphibians were collected from natural ponds and cattle ponds to compare the endemic nature of ranavirus. Hundreds of tadpoles and juvenal frogs were collected for tissue biopsy. The tissues were enzymatically digested and DNA preferentially purified. Spectral analysis revealed pure, good-quality DNA purification and ready for PCR. We performed some ranavirus-spiked controls to determine our minimum detection limits and tested for PCR-inhibition in all samples. Finally, a collection of samples were DNA sequenced for final confirmation that what we were seeing was the actual viral DNA.
Chatfield, M. W. H., B. B. Rothermel, C. Brooks, and J. Kay. 2009. Detection of Batrachochytrium dendrobatidis in amphibians from the Great Smoky Mountains of North Carolina and Tennessee, USA. Herpetological Review 40(2), 176-179.
- In this project, amphibians were captured/released and swabbed from the Great Smoky Mountains. The swabs were analyzed by PCR (and qRTPCR) the presence of chytrid fungi DNA. We grow the chytrid fungi in the lab, so we are able to do all sorts of controls. The data revealed that we could detect chytrid fungi DNA down to just 30 zoospores per sample (typical sample ~2mL volume).
We are interested in partnering with you on your research projects. We are sure you will find our lab has the expertise and experience for cost effective DNA detection methodologies for your specific research project. Please contact us for a consultation.
Dr. Chad Brooks
Austin Peay State University
Department of Biology
Sundquist Science Center
681 Summer Street
Clarksville, TN 37040